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Host-Fungus Interactions
Methods and Protocols, Methods in Molecular Biology 845
ISBN/EAN: 9781617795381
Umbreit-Nr.: 1262235
Sprache:
Englisch
Umfang: xvi, 597 S., 67 s/w Illustr., 37 farbige Illustr.,
Format in cm:
Einband:
gebundenes Buch
Erschienen am 10.02.2012
Auflage: 1/2012
- Zusatztext
- Microbiologists, medical mycologists, immunologists, and biochemists are increasingly working together to focus on the processes involved in the progression and treatment of fungal disease. Host-Fungus Interactions: Methods and Protocols is designed for research scientists who are involved in this work and interested in undertaking new or comparative studies of interactions between the mammalian host and clinically important fungal pathogens. Aiming to combine approaches for reverse genetics in pathogenic fungi with methods for their application in in vitro and in vivo models of disease, the book includes methods for the culture and genetic manipulation of the primary fungal pathogens and the opportunistic pathogens, as well as methods for investigating host-fungus interactions in model systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Host-Fungus Interactions: Methods and Protocols describes available molecular methods and fungal infection models in great detail in order to encourage researchers to try new approaches to investigating host-fungus interactions with added levels of confidence.
- Kurztext
- Examines the culture and genetic manipulation of primary fungal pathogens as well as interactions in model systemsIncludes detailed, tested methods that are ready for the labFeatures key tips and implementation advice necessary to achieve successful results
- Leseprobe
- InhaltsangabePart I: Gene Disruption 1. Gene Deletion in Candida albicans Wild-Type Strains Using the SAT1-Flipping Strategy Christoph Sasse and Joachim Morschhäuser 2. Mini-Blaster-Mediated Targeted Gene Disruption and Marker Complementation in Candida albicans Shantanu Ganguly and Aaron P. Mitchell 3. Rapid Detection of Aneuploidy Following the Generation of Mutants in Candida albicans Megan D. Lenardon and André Nantel 4. Agrobacterium-Mediated Insertional Mutagenesis in Histoplasma capsulatumi Olga Zemska and Chad A. Rappleye 5. Targeted Gene Disruption in Cryptococcus neoformans Using Double Joint PCR with Split Dominant Selectable Markers Min Su Kim, Seo-Young Kim, Kwang-Woo Jung, and Yong-Sun Bahn 6. Multiple Gene Deletion in Cryptococcus neoformans Using the Cre--lox System Lorina G. Bakerand and Jennifer K. Lodge 7. Gene Disruption in Aspergillus fumigatus Using a PCR-Based Strategy and In Vivo Recombination in Yeast Iran Malavazi and Gustavo Henrique Goldman 8. Targeted Gene Deletion in Aspergillus fumigatus Using the Hygromycin-Resistance Split-Marker Approach Fabrice N. Gravelat, David S. Askew, and Donald C. Sheppard 9. Gene Disruption in Coccidioides Using Hygromycin or Phleomycin Resistance Markers Chiung-Yu Hung, Hua Zhang Wise, and Garry T. Cole Part II: Modulation of Gene Expression: RNAi Gene Knockdown 10. RNAi-Based Gene Silencing Using a GFP Sentinel System in Histoplasma capsulatum Brian H. Youseff and Chad A. Rappleye 11. RNA Interference in Cryptococcus neoformans Michael L. Skowyra and Tamara L. Doering 12. Gene Knock-Down in Paracoccidioides brasiliensis Using Antisense RNA João F. Menino, Agostinho J. Almeida, and Fernando Rodrigues Part III: Modulation of Gene Expression: Regulatable Promoters 13. Tetracycline-Inducible Gene Expression in Candida albicans Michael Weyler and Joachim Morschhäuser 14. Galactose-Inducible Promoters in Cryptococcus neoformans var. grubii Lorina G. Baker and Jennifer K. Lodge 15. Modular Gene Over-Expression Strategies for Candida albicans Vitor Cabral, Murielle Chauvel, Arnaud Firon, Mélanie Legrand, Audrey Nesseir, Sophie Bachellier-Bassi, Yogesh Chaudhari, Carol Munro, and Christophe d'Enfert Part IV: Responses to Infection In Vivo 16. Interactions Between Macrophages and Cell-Wall Oligosaccharides of Candida albicans Héctor M. Mora-Montes, Christopher McKenzie, Judith M. Bain, Leanne E. Lewis, Lars P. Erwig, and Neil A.R. Gow 17. Murine Bone Marrow-Derived Dendritic Cells and T Cell Activation by Candida albicans Joanne Gibson, Neil A.R. Gow, and Simon Y.C. Wong 18. Phagocytosis and Intracellular Killing of Candida albicans by Murine Polymorphonuclear Neutrophils Alieke G. Vonk, Mihai G. Netea, and Bart Jan Kullberg 19. Human Oral Keratinocytes: A Model System to Analyze Host-Pathogen Interactions Torsten Wöllert, Christiane Rollenhagen, George M. Langford, and Paula Sundstrom 20