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Transgenic Maize
Methods and Protocols, Methods in Molecular Biology 526
ISBN/EAN: 9781617379352
Umbreit-Nr.: 1803660
Sprache:
Englisch
Umfang: ix, 188 S.
Format in cm:
Einband:
kartoniertes Buch
Erschienen am 19.11.2010
Auflage: 1/2010
- Kurztext
- As increasing global population and continuing economic development ensure the need for further production and cultivation of maize, the necessity of the application of transgenic technology to this model species and crop plant grows steadily. In Transgenic Maize: Methods and Protocols, experts in the various disciplines of the field contribute readily reproducible protocols covering such topics as transformation methods, transgenic maize in research, the analysis of transgenic plants, and breeding with transgenes. Written in the highly successful Methods in Molecular Biology series format, the chapters present brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Cuttingedge and easy to use, Transgenic Maize: Methods and Protocols will greatly aid researchers wishing to use these procedures in their own laboratories, and will provide readers with a better understanding of the experiments being performed by cooperators or feeforservice labs.
- Autorenportrait
- InhaltsangabePart I: Introduction 1. Design of Gene Constructs for Transgenic Maize Dong Liu Part II: Transformation Methods 2. Transient Expression of GFP in Immature Seed Tissues Colin T. Shepherd 3. Biolistic Gun Mediated Maize Genetic Transformation Kan Wang and Bronwyn Frame 4. Agrobacterium-Mediated Maize Transformation: Immature Embryos Versus Callus Vladimir Sidorov and David Duncan 5. WhiskersMediated Maize Transformation Joseph F. Petolino and Nicole L. Arnold Part III: Transgenic Maize in Research 6. Methods for Generation and Analysis of Fluorescent Protein Tagged Maize Lines Amitabh Mohanty, Yan Yang, Anding Luo, Anne Sylvester, and David Jackson 7. Use of Transgene-Induced RNAi to Regulate Endogenous Gene Expression Karen M. McGinnis 8. Plasmid Rescue: Recovery of Flanking Genomic Sequences from Transgenic Transposon Insertion Sites GuoLing Nan and Virginia Walbot Part IV: Analysis of Transgenic Plants 9. NonRadioactive Genomic DNA Blots for Detection of Low Abundant Sequences in Transgenic Maize GuoLing Nan and Virginia Walbot 10. TissuePrint ImmunoDetection of Transgene Products in Endosperm for HighThroughput Screening of Seeds M. Paul Scott 11. Determination of Transgene Copy Number by Real-Time Quantitative-PCR Colin T. Shepherd, Adrienne N. Moran Lauter, and M. Paul Scott Part V: Breeding with Transgenes 12. Herbicide Resistance Screening Assay Joan M. Peterson 13. Characterizing Transgene Inheritance Earl H. Bicar 14. Backcross Breeding Karla E. Vogel Appendix A: Sample APHIS Notification of Release of Regulated Material Appendix B: Sample SOP for Field Release