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Hemoglobin Disorders
Molecular Methods and Protocols, Methods in Molecular Medicine 82
ISBN/EAN: 9781617372780
Umbreit-Nr.: 988972
Sprache:
Englisch
Umfang: xx, 300 S., 64 s/w Illustr.
Format in cm:
Einband:
kartoniertes Buch
Erschienen am 09.11.2010
Auflage: 1/2003
- Zusatztext
- InhaltsangabeX-ray Crystallography of Hemoglobins Martin K. Safo and Donald J. Abraham Analysis of Hemoglobins and Globin Chains by High-Performance Liquid Chromatography Henri Wajcman Purification and Molecular Analysis of Hemoglobin by High-Performance Liquid Chromatograpy Belur N. Manjula and Seetharama A. Acharya Oxygen Equilibrium Measurements of Human Red Blood Cells Jean Kister and Henri Wajcman Measurement of Rate Constants for Reactions of O2, CO, and NO with Hemoglobin John S. Olson, Erin W. Foley, David H. Maillett, and Eden V. Paster Electrophoretic Methods for Study of Hemoglobins Henri Wajcman DNA Diagnosis of Hemoglobin Mutations John M. Old. Methods for Analysis of Prenatal Diagnosis, John M. Old Hemoglobin Fluorescence Rhoda Elison Hirsch Nucleation and Crystal Growth of Hemoglobins: The Case of HbC Peter G. Vekilov, Angela Feeling-Taylor, and Rhoda Elison Hirsch Semisynthesis of Hemoglobin Seetharama A. Acharya and Sonati Srinivasulu bGlobinlike Gene Cluster Haplotypes in Hemoglobinopathies Shanmugakonar Muralitharan, Rajagopal Krishnamoorthy, and Ronald L. Nagel Transgenic Mice and Hemoglobinopathies Mary E. Fabry, Eric E. Bouhassira, Sandra M. Suzuka, and Ronald L. Nagel Recombinant Single Globin-Chain Expression and Purification Kazuhiko Adachi Nuclear Magnetic Resonance of Hemoglobins Jonathan A. Lukin and Chien Ho Solubility Measurement of the Sickle Polymer Mary E. Fabry, Seetharama A. Acharya, Sandra M. Suzuka, and Ronald L. Nagel Index
- Kurztext
- The recent announcement that sickle-cell anemia and thalassemia have been corrected by the transplantation of stem cells bodes well for the future of gene therapy in hemoglobinopathies. In Hemoglobin Disorders: Molecular Methods and Protocols, Ronald Nagel, MD, has assembled a collection of readily reproducible techniques essential to the continued advance of our molecular understanding of these diseases. The book's richly experienced authors detail methods utilizing a wide variety of the latest analytical techniques, including X-ray crystallography, high performance liquid chromatography, electrophoresis, and nuclear magnetic resonance. Additional methods are offered for prenatal diagnostic analysis, the DNA diagnosis of hemoglobin mutations, hemoglobin fluorescence, and the semisynthesis of hemoglobin. Each protocol includes an introduction explaining the basic science, step-by-step instructions for its successful execution, notes on pitfalls to avoid, and tips on how to employ it effectively with novel systems and conditions. Stateoftheart and highly practical, Hemoglobin Disorders: Molecular Methods and Protocols reviews all the basic topics and techniques in this critically important field, and offers today's most comprehensive set of proven protocols for successful experimental and clinical work on hemoglobin diseases.