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Cytotoxic Drug Resistance Mechanisms
Methods in Molecular Medicine 28
ISBN/EAN: 9781617370939
Umbreit-Nr.: 984312
Sprache:
Englisch
Umfang: xi, 238 S.
Format in cm:
Einband:
kartoniertes Buch
Erschienen am 09.11.2010
Auflage: 1/1999
- Zusatztext
- InhaltsangabeDrug Resistance: The Clinical Perspective D. Alan Anthoney and Stanley B. Kaye Cell Sensitivity Assays: Clonogenic Assay Jane A. Plumb Cell Sensitivity Assays: The MTT Assay Jane A. Plumb Cell Sensitivity Assays: Detection of Apoptotic Cells In Vitro Using the TUNEL Assay Neil A. Jones and Caroline Dive Analysis of Apoptosis in Tissue Sections Vicki Save, Philip J. Coates, and Peter A. Hall Measurement of P-glycoprotein Function Henk J. Broxterman Measuring MDR-1 by Quantitative RT-PCR Susan E. Bates, Zhirong Zhan, Joanna Regis, and Erick Gamelin Microtiter Plate Technique for the Measurement of Glutathione in Fresh and Cryopreserved Lymphoblasts Using the Enzyme Recycling Method Pamela R. Kearns and Andrew G. Hall Measurement of Reduced Glutathione Using High Pressure Liquid Chromatography Linda A. Hogarth, Celia M. A. Rabello, and Andrew G. Hall Topoisomerase I and II Activity Assays Philippe Pourquier, Glenda Kohlhagen, Li-Ming Ueng, and Yves Pommier 5Fluorouracil Metabolizing Enzymes Howard L. McLeod, Lesley H. Milne, and Stephen J. Johnston Measuring DNA Adducts by Immunoassay (ELISA) Michael J. Tilby Measuring Drug-DNA Adducts in Individual Cells Adrian J. Frank Measurement of Drug-Induced DNA Interstrand Crosslinking Using the Single Cell Electrophoresis (Comet) Assay Victoria J. Spanswick, Janet M. Hartley, Timothy H. Ward, and John A. Hartley PCR Analysis of Microsatellite Instability Gillian L. Hirst O 6AlkylguanineDNA Alkyltransferase Assay Amanda J. Watson and Geoffrey P. Margison Analysis of the p53 Status of Tumors: An Overview of Methods Jonas Bergh Bcl2 Family Immunohistochemistry Lloyd R. Kelland and Philip J. Beale Genetic Analysis of Drug Resistance by Fluorescence In Situ Hybridization W. Nicol Keith Genetic Analysis of Drug Resistance by ReverseIn Situ Hybridization W. Nicol Keith Index
- Kurztext
- In Cytotoxic Drug Resistance Mechanisms, leading clinical and laboratory scientists describe cutting-edge methods for examining the mechanisms of cellular resistance to anticancer cytotoxics in human tumors. The protocols contain detailed instructions and extensive troubleshooting tips that allow researchers effectively to study a wide variety of drug resistance mechanisms, including aspects of drug-induced cell death, drug uptake/efflux, drug metabolism, and DNA repair. Each method is designed to help identify the correlation between molecular and biochemical data and the clinical responses of the patient. Cytotoxic Drug Resistance Mechanisms makes it possible to test established and laboratory-derived hypotheses with clinically derived tumor samples. State-of-the-art and readily reproducible, the methods presented here afford basic and clinical scientists a powerful complement of tools for investigating all the clinically relevant mechanisms used as markers of the biological response to anticancer chemotherapeutics today.