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Listeria monocytogenes
Springer Protocols, Methods in Molecular Biology 1157
ISBN/EAN: 9781493907021
Umbreit-Nr.: 6246679
Sprache:
Englisch
Umfang: x, 286 S., 25 s/w Illustr., 17 farbige Illustr., 2
Format in cm: 1.7 x 26 x 18.5
Einband:
gebundenes Buch
Erschienen am 04.05.2014
Auflage: 1/2014
- Zusatztext
- InhaltsangabePart I: Detection, Quantification, and Confirmation1. Sampling the Production/Processing Environment for Listeria Anca Ioana Nicolau and Andrei Sorin Bolocan2. Traditional Methods for Isolation of Listeria monocytogenes Rui Magalhães, Cristina Mena, Vânia Ferreira, Gonçalo Almeida, Joana Silva, and Paula Teixeira3. Confirmation of Isolates of Listeria by Conventional and Real-Time PCR David Rodríguez-Lázaro and Marta HernándezPart II: Characterization and Typing4. Serotype Assignment by Sero-Agglutination, ELISA, and PCR Lisa Gorski5. Pulsed-Field Gel Electrophoresis (PFGE) Analysis of Listeria monocytogenes Marion Dalmasso and Kieran Jordan6. Multi-Locus Sequence Typing (MLST) of Listeria monocytogenes Beatrix Stessl, Irene Rückerl, and Martin Wagner7. Ribotyping and Automated Ribotyping of Listeria monocytogenes Mazin Matloob and Mansel Griffiths8. Fluorescent Amplified Fragment Length Polymorphism (fAFLP) Analysis of Listeria monocytogenes Corinne Amar9. High-Throughput Characterization of Listeria monocytogenes Using the OmniLog Phenotypic Microarray Edward M. Fox and Kieran Jordan10. Analysis of Listeria monocytogenes Subproteomes Michel Hébraud11. The Listeria Cell Wall and Associated Carbohydrate Polymers Marcel R. Eugster and Martin J. Loessner12. Use of Bacteriophage Cell Wall Binding Proteins for Rapid Diagnostics of Listeria Mathias Schmelcher and Martin J. Loessner13. Virulence Characterization of Listeria monocytogenes Swetha Reddy and Mark L. Lawrence14. Internalization Assays for Listeria monocytogenes Andreas Kühbacher, Pascale Cossart, and Javier Pizarro-CerdáPart III: Strain Manipulation15. Extraction and Analysis of Plasmid DNA from Listeria monocytogenes Aidan Casey and Olivia McAuliffe16. Generation of Non-Polar Deletion Mutants in Listeria monocytogenes Using the "SOEing" Method Kathrin Rychli, Caitriona M. Guinane, Karen Daly, Colin Hill, and Paul D. Cotter17. Mutant Construction and Integration Vector-Mediated Gene Complementation in Listeria monocytogenes Reha Onur Azizoglu, Driss Elhanafi, and Sophia Kathariou18. Absolute and Relative Gene Expression in Listeria monocytogenes Using Real Time PCR Roberta Mazza and Rina Mazette19. Genome Sequencing of Listeria monocytogenes Stephan Schmitz-Esser and Martin Wagner20. Using Enhanced Green Fluorescent Protein (EGFP) Promoter Fusions to Study Gene Regulation at Single Cell and Population Levels Marta Utratna and Conor P. O'ByrnePart IV: Control Methods21. Control of Listeria monocytogenes in the Processing Environment by Understanding Biofilm Formation and Resistance to Sanitizers Stavros G. Manios and Panagiotis N. Skandamis22. Vaccination Studies: Detection of a Listeria monocytogenes Specific T Cell Immune Response Using the ELISPOT Technique Mohammed Bahey-El-Din and Cormac G.M. Gahan23. Sampling the Food Processing Environment: Taking up the Cudgel for Preventive Quality Management in Food Processing Environments Martin Wagner and Beatrix Stessl
- Kurztext
- Listeria monocytogenes is still a major threat to public health. A new book in the Methods in Molecular Biology series, Listeria monocytogenes: Methods and Protocols addresses its titular pathogen with protocols and methodologies used in research to gain a better understanding of Listeria at a molecular level. The topics covered include sampling in order to isolate Listeria, methods for their identification and characterization, methods for gene manipulation, and, finally, methods for control of the organism. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Vital and authoritative, Listeria monocytogenes: Methods and Protocols aims to contribute to the harmonization of the methods used in the field and will therefore benefit all those interested in Listeria research.